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Routine sample preparation and HPLC analysis for ascorbic acid (vitamin C) determination in wheat plants and Arabidopsis leaf tissues

机译:用于小麦植物和拟南芥叶片组织中抗坏血酸(维生素C)测定的常规样品制备和HPLC分析

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摘要

Plants have developed various mechanisms to protect themselves against oxidative stress. One of the most important non-enzymatic antioxidants is ascorbic acid. There is thus a need for a rapid, sensitive method for the analysis of the reduced and oxidised forms of ascorbic acid in crop plants. In this paper a simple, economic, selective, precise and stable HPLC method is presented for the detection of ascorbate in plant tissue. The sensitivity, the short retention time and the simple isocratic elution mean that the method is suitable for the routine quantification of ascorbate in a high daily sample number. The method has been found to be better than previously reported methods, because of the use of an economical, readily available mobile phase, UV detection and the lack of complicated extraction procedures. The method has been tested on Arabidopsis plants with different ascorbate levels and on wheat plants during Cd stress.\ud
机译:植物已经开发出多种机制来保护自己免受氧化胁迫。最重要的非酶抗氧化剂之一是抗坏血酸。因此,需要一种快速,灵敏的方法来分析农作物中抗坏血酸的还原和氧化形式。本文提出了一种简单,经济,选择性,精确和稳定的HPLC方法来检测植物组织中的抗坏血酸。灵敏度高,保留时间短和简单的等度洗脱意味着该方法适用于每天大量样品中抗坏血酸的常规定量分析。已经发现该方法比以前报道的方法更好,这是因为使用了经济,易于获得的流动相,UV检测以及缺乏复杂的提取程序。该方法已在不同抗坏血酸水平的拟南芥植物和镉胁迫下的小麦植物上进行了测试。\ ud

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